Only one seeded player remains in the women's draw of the Bendigo International after two more big guns crashed out on Wednesday.
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Number one seed Cristina Bucsa lost 6-2, 6-4 to the impressive Yuliya Hatouka from Belarus, while eighth-seeded American Jamie Loeb suffered a 6-3, 6-3 loss to Mexican Fernanda Contreras Gomez.
With two round of 16 games still to be played on Thursday, the only seeded player to qualify for Friday's quarter-finals is sixth-seed CoCo Vandeweghe, who survived a tough three-set clash with fellow American Hanna Chang.
Vandeweghe now plays Hatouka in the quarter-finals, while Contreras Gomez will tackle Ysaline Bonaventure after the Belgian player disposed of qualifier Whitney Osuigwe 6-3, 6-1.
Liechtenstein's Kathinka von Deichmann hasn't dropped a set through her first two matches.
She'll face Russian Elina Avanesyan in the quarters. Avanesyan defeated countrywoman Valeria Savinykh in a tight three-set match on Wednesday.
The remaining quarter-final participants will be decided on Thursday.
Andorra's Victoria Jimenez Kasintseva plays Bosnia and Herzegovina's Dea Herdzelas, while Switzerland's Joanne Zuger will meet Arianne Hartono from the Netherlands. Both matches start at 10am on Thursday.
France and Italy dominate the final 16 of the men's tournament.
Four Frenchmen and four Italians will compete on Thursday for a place in Friday's quarter-finals.
The most intriguing matches in the final 16 will be played on centre court, starting with the battle between 10th seed Enzo Couacaud of France and Italian Thomas Fabbiano.
Just 25 places splits the two players in the world rankings and they're yet to play each other on tour.
Fabbiano hasn't dropped a set in his two matches, while Couacaud survived a tough a three-set match in round two against Argentina's Nicolas Kicker.
The second match on centre court is between giant-killing German Matthias Bachinger and Italy's Franco Agamenone.
Bachinger knocked out number one seed Nikola Milojevic in the second round.
The third match on centre court sees American eighth-seed Ernesto Escobedo plays ninth-seed Jason Jung from Taipei.
Escobedo, 25, holds a 2-1 career head-to-head avantage on Jung.
The highest-ranked seed remaining in the tournament - third-seeded Frenchman Hugo Grenier - plays 16th seed Zizou Bergs of Belgium in the second match on court one. Grenier moved his way into the last 16 on the back of a 7-5, 6-0 win over Lorenzo Giustino.
Fourth-seed Quentin Halys plays French countryman Mathias Bourgue in the opening match on court one from 10am.
One of the highlights of Wednesday's men's matches was Portugal's Gastao Elias upset win over 12th seed Blaz Rola, 6-4, 3-6, 6-3.
Fifth seed Salvatore Caruso rallied from one set down to edge out Alexandre Muller 2-6, 6-2, 6-1.
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