The Bendigo Blood Donor Centre saw over 40 per cent of its donors cancel or reschedule their donations last month with nine per cent failing to turn up on the day.
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Lifeblood spokesperson Shae Burns said the demand for blood has been at its highest in over a decade.
She said now only one in every two appointments are being kept, with a third cancelled or rescheduled on the day.
"Australians are enjoying holidays, catching up with friends and family, and getting back to every day outings, but the need for blood never stops," she said.
"In fact, annual demand for blood is at its highest level in over a decade, with hospitals now using four per cent more blood than they did compared to the last two years.
"We understand that plans change so if you do need to cancel or reschedule, please contact Lifeblood as early as possible so we can find someone to take your place."
Australian Red Cross Lifeblood released figures showing last-minute blood donation cancellations left 44,000 donor couches empty last month, the equivalent of some football stadiums.
Ms Burns said between now and June 1, regional Victoria will need more than 1000 additional blood donors.
"There is no substitute for blood and we need every type, every day, to meet the needs of Australian patients who depend on it," she said.
"There are always people in hospitals needing blood for cancer treatment, surgery, blood disorders and obstetrics.
Ms Burns said the Bendigo Blood Donor Centre will be opening a special session on Sunday May 30 to make it easier for people to donate.
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"We just want to let everyone know that if you are between 18 and 75, feeling well, you may be able to donate, give us a call, book an appointment, download the app," she said.
Donors can check their eligibility to donate blood online.
To book a donation please call 13 14 95, click here or download the free Donate Blood app.
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