RSPCA Victoria have seized 75 roosters allegedly being groomed for cockfighting, from a property near Malmsbury.
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Working with Victoria Police, Macedon Ranges Shire Council and Brimbank City Council, almost 200 birds were seized during the raid.
As well as the 75 roosters, RSPCA inspectors took a further 119 birds including chicks, mature hens and juvenile roosters.
The property owner is currently banned from being in charge of poultry following a prior prosecution. They are eight years into a 10 year ban
"It is a shocking reality that cockfighting, a barbaric activity, continues to operate in Victoria," RSPCA Victoria inspectorate team leader Karen Collier said.
"The successful removal of the birds was achievable due to the joint efforts between all four organisations and resulted in a step forward for animal welfare in Victoria."
Macedon Ranges Shire director of assets and operations Shane Walden said council officers played key roles leading up to the execution of RSPCA's warrants.
"Cockfighting is an abhorrent and cruel activity leading to severe suffering of the roosters involved," he said.
"Council takes a zero tolerance approach to animal fighting and works closely with RSPCA and Victoria Police to stamp out any identified cruelty to animals.
"Council is continuing investigations into conditions and structures at the property."
Seven RSPCA inspectors, two veterinarians and one animal attendant assessed and help remove the birds from the property.
RSPCA inspectors also found cockfighting implements at the property.
Cockfighting is illegal in Australia. It is also illegal to possess any equipment designed for cock fighting.
Anyone found guilty keeping or using a premises for animal fighting, or of encouraging animals to fight, can receive two years prison or a fine of up to $82,610. Fines of up to $3304 can be imposed for possessing cockfighting equipment.
The investigation is ongoing.