Bendigo-based Department of Transport staff will move into the new Bendigo GovHub when it is completed.
The move will bring 115 VicRoads, Regional Roads Victoria and Public Transport Victoria staff from their current site in East Bendigo as the newest GovHub tenants.
Bendigo's GovHub is expected to house 1000 staff with 400 desks set aside for City of Greater Bendigo staff and 600 being made up of state government department staff.
Regional Development Victoria, Agriculture Victoria and Parks Victoria will also be tenants in the GovHub.
Regional Roads Victoria's northern regional director Brian Westley said the Department of Transport's move to the GovHub was exciting.
He said their current East Bendigo site is at capacity.
"Our lease ends in 2022 but the opportunity to be co-located with other agencies is what we're most excited about," he said.
"Not only is (the GovHub) a boon for Bendigo and the broader community it also means we see state and local government agencies bought together.
"Bendigo is growing at phenomenal rate as is the broader city of Greater Bendigo. It's important (we are) planning outcomes not just for today but for 10, 20 and 30 years time.
"Being co-located with the Department of Land, Water and Planning and the City of Greater Bendigo planners means we can get far better outcomes (for the community)."
Minister for Transport Infrastructure Jacinta Allan said the co-location of services would benefit the city.
"It brings great economical benefit as well as great service benefit," she said.
"That co-location of services will provide a much better way for the local community to access services whether its paying rates or getting advice on planning, that can be provided in one place.
"We're going to see a building that accommodates 1000 people who will work in heart of Bendigo CBD, which will bring more activity to (central Bendigo) and support the local businesses and traders."
Ms Allan also announced two new state government agencies will take up residence in the GovHub.
The Portable Long Service Authority and the Victorian Labour Hire Licensing Authority creating 55 new jobs.
"We wanted to add jobs and the Long Service Authority and the Victorian Labour Hire Licensing Authority are new state government agencies and we made the decision to locate them in Bendigo as part of the GovHub," Ms Allan said.
City of Greater Bendigo director of corporate performance Andrew Cooney said the one-stop shop aspect of the GovHub would be good for both customers and government agencies.
"(Having) our staff located with staff from (state departments) has benefits for us and the community," he said.
Construction on the Bendigo GovHub is expected to start in 2020 and be completed by 2022.
The state government committed $16 million to the project in the 2018 budget with the balance of the $90 million project to come through private investment.
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