Police are investigating after tools and computer equipment were stolen from Windarring in Flora Hill.
Offenders broke into the facility between Sunday night and Monday morning. The main doors to the building were damaged as they broke in.
Windarring is disability services facility run out of the YWCA building on Townsend Street.
Police say they have no suspects at this stage.
Anyone with information about the burglary should contact Bendigo Station on 5448 1300 or Crime Stoppers on 1800 333 000.