The Bendigo mates will next month run 100 kilometres through Victoria's High Country to raise awareness and funds for the condition which affects one in 100 Australian children.

About 100 teams of two will tackle the Oscars 100 Hut2Hut on February 17-19, which will support Mansfield Autism Statewide Services, an independent organisation assisting young people living with autism and their families.

Edwards, a social worker, said the decision to tackle the inaugural endurance challenge was an easy one to make.

"When you see the battle families living with autism go through every day, running 100 kilometres is not much of a battle," he said.

"Obviously it will be a physical and mental challenge for Dan and I, but it's nothing compared to the challenge of autism.

The trek through Mount Buller will involve 5700 metres of climbing.

It will be Edwards' first attempt at the 100km distance, while O'Bree, a policeman, has done it before.

The run has a 55-hour cut-off, with runners able to stop for sleep or food breaks at two designated hut checkpoints.

Edwards and O'Bree intend completing the course in one hit.

"You have to get to a certain point, 70 kilometres, in 15 hours," O'Bree said.

"If you don't get to that point you have to stay the night, but we're not keen on staying overnight."

Preparing for the run has not been an issue for both men.

They train all-year round and regularly compete in marathons, most recently in October.

"We train together at least once a week as well as doing our own thing," Edwards said.

"We've been running up Mount Alexander a fair bit, trying to make it as close to the real thing as possible.

"But I think Mount Buller might be a lot harder than Mount Alexander."

Edwards and O'Bree have so far raised $5000 and hope to boost that significantly with a fundraiser at the Axedale Tavern in February.

"Percentages of the meals will be donated to us and there will be a bit of auction as well," O'Bree said.

"Hopefully we can raise a few more pennies then. We have a few donation tins floating about Bendigo.

"People have been very generous - it's been a great response.

Bendigo mother Trudi Richardson knows first hand the difference Mansfield Autism Statewide Services make.

Her son Elliott spent one term in each of 2015 and '16 at the service's school.

"It's a really high cost and sadly it's out of the reach of some families," she said.

"It's an organisation that should be every where and accessible to everyone.

To support Edwards and O'Bree's fundraising efforts, visit https://www.mycause.com.au/page/141769/bendigo-boys-100km