Radio station 3BO raised more than $20,000 for Bendigo Health in a telethon on Friday.
Phones rang non-stop from 6am to 6pm with donations towards the Give Me 5 for Kids - a campaign that raises funds for sick children and their families.
Promotions coordinator at 3BO Jessica Elsley said their busiest donation time was between 8am and 9am.
They had 53 calls in one hour and raised more than $5000.
Ms Elsley said the average donation amount was $50 and the largest donations reached $1000.
She said there were plenty of volunteers to take calls and the mood was positive.
"They've been really excited and happy to be here," Ms Elsley said.
She said there were cheers from the volunteers whenever larger donations came through.
Ms Elsley said the preparation phase for Give Me 5 for Kids had been going for some months.
"We've been preparing since January for it," she said.
It was good though, very rewarding. I think it’ll just get bigger and better.Jessica Elsley
She was very tired after the 12-hour event but was keen to do it again.
"It was good though, very rewarding," she said.
"I think it’ll just get bigger and better."
Ms Elsley said the money would go towards resuscitation equipment, a carbon dioxide monitor and a scholarship to train nurses and doctors.
Friday's event was part of the station's major appeal running throughout June.
All money raised will go to Bendigo Health's paediatric unit which provides care to babies, children and teenagers across the Loddon Mallee region.
The station is aiming to raise a total of $125,000 by the end of June.
Last year Give Me 5 For Kids raised almost $2.5 million nationwide.
The fundraiser was launched by media company Austereo in the 1990s and has raised more than $12 million since it began.
More than 30 Austereo regional radio stations are taking part.
To donate call 13 27 10 or visit www.giveme5forkids.com.au