More than 1,200 cyclists in 73 teams will be setting off from Mildura, Echuca and Swan Hill on Saturday for the 2014 Murray to Moyne which sees cyclists riding 520 kilometres relay-style to Port Fairy over a 24-hour period.

Organisers are urging motorists travelling along the route to take care and watch out for cyclists.

M2M Coordinator Maggie Leutton said motorists would have plenty of warning of cyclists ahead.

“Drivers will encounter small groups of cyclists so if you’re planning on using the route roads over the weekend of April 5-6, look out for the M2M safety vehicles that will signal cyclists ahead or behind,” she said.

Ms Leutton said organisers were also encouraging locals to cheer the teams through their towns or along the three routes.

“Everyone has spent the past months training hard for their ride and it’s a great testament to their hard work in raising funds for their local health service,” she said.

Route maps are available on the M2M website www.murraytomoyne.com.au