More than 40 riders, including five exchange students, and a support crew of 16 will cover 600km in six days ahead of a civic reception in Bendigo on March 14 for the start of the Rotary District 9820 Conference.

Ride spokesman Barry Irving said participants would be doing loops around Eppalock, Bridegwater, Laanecoorie, Maldon and Redesdale.

"We'll be riding about 100km every day and stopping off at the schools with Harold the Giraffe from Life Education," Mr Irving said.