BENDIGO V/Line services will not stop in Melbourne on Saturday morning as Victoria Police shuts down the public transport system.
Trains or replacement coach services from Bendigo will instead stop at Sunbury between 8am and 2pm on September 18.
Chief Commissioner Shane Patton said all public transport would bypass the Melbourne CBD in an effort to prevent a planned anti-lockdown protest.
"Anyone who's planning to come in, it is an illegal gathering and we'll be doing everything we can to prevent that gathering," Mr Patton said.
"And if people do get to make it into the CBD for that gathering, we'll be enforcing that. We'll be doing everything we can to prevent access to the city."
There will also be major road closures leading into the Melbourne CBD. Access will only be granted at roadblocks to those travelling for essential work, healthcare, or to attend a vaccination appointment.
Those conducting authorised travel are urged to allow plenty of extra time for their journeys and must carry identification and proof of their reason for travel.
Services will resume from 2pm onward, however it is expected to take some time to resume the regular timetable.
"We understand that this will be inconvenient and will cause disruption to a large number of people," a department of transport spokesperson said.
"However, the health and safety of the Victorian community is paramount, so we ask people to do the right thing and only travel for the permitted reasons."
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