Qantas will stand down about 2500 employees for an estimated two months.
The business is yet to confirm whether that will include staff on Bendigo to Sydney flights.
It has so far said it is a measure to deal with a "significant drop" in flying caused by Greater Sydney restrictions.
"Today's decision will directly impact domestic pilots, cabin crew and airport workers, mostly in New South Wales but also in other states given the nature of airline networks," the company said.
"Employees will be given two weeks' notice before the stand down takes effect, with pay continuing until mid-August."
The new shutdown has come as the company continues to recover from lockdowns that grounded its domestic and international fleet and forced 20,000 workers to stand down.
Qantas has welcomed government disaster payments it says will be key to helping eligible employees get through the shutdown.
The company does not expect job losses.
Qantas chief Executive Alan Joyce said the stand downs reflected the same reality confronting many businesses.
"This is clearly the last thing we want to do, but we're now faced with an extended period of reduced flying and that means no work for a number of our people," he said.
"We've absorbed a significant amount of cost since these recent lockdowns started and continued paying our people their full rosters despite thousands of cancelled flights."
Mr Joyce said the vaccine rollout represented a light on the horizon for the embattled company.
Australia just needs more people rolling up their sleeves as more vaccine arrives," he said.
"The challenge around opening international borders remains.
"There are still several thousand Qantas and Jetstar crew who normally fly internationally and who have been on long periods of stand down since the pandemic began. Higher vaccination rates are also key to being able to fly overseas again, and finally getting all our people back to work."
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