Coliban Water incident manager Christine Grundy said the clean-up and disinfection efforts are nearing completion.
"The spill has not impacted on stormwater drains," she said. "The clean-up and disinfection process took place this afternoon with the help of two suction trucks.
Coliban Water will be investigating the cause of the incident."
Efforts to clear a major Epsom intersection will take two hours longer than previously anticipated.
Posting to social media, Coliban Water said the clean-up and disinfection process was estimated to be complete by 3.30pm.
"Crews are working to lighten the load of the dislodged trailer to allow it to be removed from the road," the post said.
"Please be aware of continued traffic delays in this area. Thank you for your patience."
Coliban Water says the biosolids that spilled onto the Midland Highway at Epsom this morning were produced as part of the wastewater treatment process.
It says it will investigate why the trailer dislodged from the truck, causing the spill.
"The biosolids being transported today were destined for the Elmore Wastewater Reclamation Plant," Coliban Water said in a social media post.
"Biosolids contain nutrients which mean that, after storing them for approximately three years, they can be reused in line with EPA guidelines as farm fertiliser or soil conditioner to improve growth."
Coliban Water expected the clean-up to be completed by 1.30pm.
The faecal matter that spilled on a major Epsom intersection is believed to have come from a treatment plant.
It is not believed to be hazardous.
Coliban Water, the Environment Protection Authority and the City of Greater Bendigo are among the agencies responding.
Police are managing traffic in the area.
Coliban Water has been contacted for comment.
Emergency services are responding after a double trailer separated from a rig at Epsom.
Firefighters were called to help with cleaning up after faecal matter spilled onto the road at the intersection of the Midland Highway and Howard Street.
The call came through shortly after 10.30am.
A spokesperson for the Country Fire Authority said water agencies were being notified.
More to come.
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