Greater Bendigo remains at zero active coronavirus cases according to data from the Department of Health and Human Services.
Central Victoria has two active cases remaining, both in the Macedon Ranges Shire.
Mount Alexander, Campaspe, Loddon, Central Goldfields, Gannawarra and Buloke shires have no active cases.
Buloke Shire has not recorded a COVID-19 cases since the pandemic began.
One new case is listed in regional Victoria, with the Mitchell Shire recording it.
The Mitchell Shire, which borders Greater Bendigo near Heathcote, has had 85 cases in total since the pandemic began and now has one active case.
Victoria premier Daniel Andrews has confirmed regional Victoria now has 10 active COVID-19 cases remaining.
There has been one new case in regional Victoria since yesterday.
Across the state there are 444 active cases remaining.
Mr Andrews said there were 16,027 test results since yesterday, which the premier said was a strong number.
He flagged the importance of testing as he prepares to make announcements relating to easing restriction, particularly in metropolitan Melbourne
"It's a big part of us being able to get to COVID normal," he said.
Regional Victoria's 14-day rolling average is at 0.8. It is is 23.6 in Melbourne.
"In order to take the next step (in Melbourne) we are targeting an average of 30 to 50 cases (over 14 days)," Mr Andrews said.
"I will be back here tomorrow and will make annoucnements that are proof positive this strategy is working.
"Ultimately, it is about getting numbers down, perhaps a little faster than what we thought. It's a credit to every Victorian following the rules."
Mr Andrews also announced minister Martin Foley would be sworn in as health minister this afternoon following the resignation of Jenny Mikakos this morning.
Mr Foley will become the Minister for Health, Minister for Ambulance Services and Minister for the Coordination of Health and Human Services COVID-19.
"Jenny resigned this morning, which I believe is the appropriate course of action to take," Mr Andrews said. "I thank her for her dedicated service and hard work. We are grateful to her for contributions to parliament, the people of Victoria and our party.
"I have asked Martin Foley to become health minster and he will be sworn in following this media conference.
"Other arrangements and portfolio changes will be announced at the appropriate times. Martin is well equipped to be at the forefront of the fight against this virus."
Victoria's number of new coronavirus cases have remained steady with 12 new cases recorded over night.
It brings the state's total number of cases to 20,130 since the pandemic began.
The overnight data from the Department of Health and Human Services also included the loss of one life.
The number of lives lost to coronavirus in Victoria is now at 782.
Regional Victoria's 14-day rolling average is at 0.8 with no mystery cases in the past 14 days. Melbourne's rolling average is 23.6.
Yesterday, regional Victoria had 11 active cases with DHHS data showing no active cases in Bendigo for the first time since June.
There are two active cases remaining in the Macedon Ranges Shire and none in Mount Alexander, Campaspe, Loddon, Central Goldfields, Buloke or Gannawarra.
Bendigo leader's celebrated the news but cautioned against people becoming complacent against the virus.
The news was tempered with Bendigo Health saying it is treating a patient with coronavirus.
The patient is not a resident of Greater Bendigo and not included in the local government area's statistics.
Bendigo Health also issued a warning for people who visited the Heathcote IGA on September 20 and 21 after a person with COVID-19 visited there on those dates.
Anyone who visited the supermarket on those dates is urged to monitor themselves and get tested if even mild symptoms present.
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