There are now two active cases of COVID-19 in the Macedon Ranges, after a resident contracted the virus.
Health officials said the person became infected in metropolitan Melbourne - not in the Macedon Ranges Shire - and went into isolation while awaiting their test result.
Close contacts have been notified, DHHS said.
The circumstances mean that the overall number of COVID-19 cases for the Macedon Ranges remains steady at 58.
Coronavirus cases elsewhere in central Victoria remained unchanged on Saturday.
The only other active case in the region is in Greater Bendigo.
Premier Daniel Andrews said the fact that just 21 new cases were confirmed across Victoria today was a sign that people could be "hopeful and optimistic", AAP reported.
"The 14-day average ... at that level, under 40, is something that every Victorian should be positive about, proud about, and very hopeful and optimistic about for the weeks ahead," Mr Andrews said.
Despite the good news, the premier said there would be no easing of restrictions in metropolitan Melbourne ahead of the September 28 timeline.
There were 21 new cases of COVID-19 confirmed in Victoria overnight, health officials said.
But the Department of Health and Human Services confirmed seven more deaths were recorded.
For regional Victoria there was positive news, with the rolling 14-day average for new cases dropping below two.
It now sits at 1.9.
Updated figures for local infections are due to be published later this afternoon.
It has been more than a week since a new case was confirmed in central Victoria.
There is one active case in Greater Bendigo, and another in the Macedon Ranges.
A program testing workers who have no symptoms of COVID-19 is being piloted in Bendigo.
Bendigo Health's rapid response team will be going to workplaces in the region and conducting saliva testing for asymptomatic workers.
The Bendigo Police Station was the first place in Victoria to take part in the program. More than 80 police officers were tested through the initiative on Friday.
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