The new two-way bike lane flows along the south side of Ellis Street in Flora Hill, meaning cyclists share the same road as motorists but not the same space.

Works to develop the bike lane started in December. Bike Bendigo president Nicola Dunnicliff-Wells said it was great to see the space finally open to the public.

"I think it's really exciting," she said. "Now that there is green paint on the lane, it really makes a difference. It makes it so much clearer and more inviting."

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Ellis Street handles traffic going to Bendigo South East College, La Trobe University's Bendigo Campus, and the Strathdale shopping precinct.

Ms Dunnicliff-Wells said the new lane would ensure cyclists were safe.

"What it means is that anybody can ride a bike on that busy stretch of road without having to mix with traffic," she said.

"What it does is give people, particularly kids, a bridge to riding on the road.

"They can become more confident and skilled so eventually they can safely ride in traffic."

Ms Dunnicliff-Wells said she hoped this new lane would be the first of many in Bendigo.

"The Ellis Street bike lane really does form a really important part of the university route from central Bendigo to La Trobe," she said.

"Williamson and Mundy Streets would been some other important links, but this is a major part of the route which is complete."