Victoria's chief health officer Brett Sutton confirmed 64 new cases of coronavirus on Tuesday, bringing the state's total to 411, with 41 of those in regional areas.
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There remain no cases recorded in the Greater Bendigo council area.
A third person in Mount Alexander Shire has tested positive after Castlemaine Health confirmed two positive coronavirus test results in Castlemaine on Monday.
There are also three cases in Mitchell Shire and two cases in Macedon Ranges Shire.
No patients have been admitted by Castlemaine Health with coronavirus, according to a spokesperson.
"Castlemaine Health continues to have no patients admitted with Covid-19 nor have we had any positive results returned for the 41 Covid-19 tests we've conducted so far," the spokesperson said.
Mr Sutton said six confirmed cases of coronavirus may have been acquired through community transmission.
There are 12 people in hospital, including two patients in intensive care.
113 people have recovered and more than 25,000 Victorians have been tested to date.
The Department of Health and Human Services follows up and monitors all close contacts of confirmed cases and provides them with information and support.
All close contacts must self isolate for 14 days.
All people arriving from any international destination must self isolate for 14 days as per Commonwealth Government direction.
Victoria Police have recently been given strong powers to enforce requests to isolate, where required.
Under the State of Emergency, people who don't comply with a directive could receive a fine of up to $20,000.
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