ANOTHER 20 bats at Rosalind Park have died from heat stress after the temperature in Bendigo soared past 40 degrees last week.
The City of Greater Bendigo closed the fernery and one of the footpaths in the park to limit the disturbance to the bats.
Fernery irrigation systems were also activated to cool down the animals.
Office of the Conservation Regulator's forest and wildlife officer Phuong Tran said about 30 grey-headed flying foxes were given oral fluids and treated with spraying. One flying fox was treated in a wildlife shelter.
"The OCR is working with the City of Greater Bendigo to ensure the welfare of the colony of grey-headed flying foxes in Rosalind Park is managed during high temperatures," Ms Tran said.
It comes after the Department of Environment, Land, Water, and Planning confirmed 170 bats were killed in a heat stress event in January.
There were about 20,000 bats at Rosalind Park in October, but Ms Tran said the population had dropped to about 700 this month.
"Grey headed flying fox populations can fluctuate rapidly," Ms Tran said.
"Environmental factors such weather conditions and food availability will influence populations such as the one in Rosalind Park.
"The colony is currently showing no signs of heat stress but DELWP are monitoring the animals."
Ms Tran urged residents to be mindful of the flying foxes.
"If you see a deceased flying fox on the ground do not touch it as they can carry disease," she said. "Please contact DELWP at 136 186, or contact your local wildlife shelter to have it removed."
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