PALL Mall will be reduced to one lane in each direction for two weeks this month to allow for tram track and drainage works.
Works are scheduled at the Williamson Street intersection from June 17 - 28.
Pall Mall will be affected between the Alexandra Fountain and Mundy Street.
"Left hand turns will be permitted into and out of Williamson Street, but right hand turns will not be allowed," the City of Greater Bendigo's engineering manager, Brett Martini, said.
There will be no impact on right hand turns at the Bull Street/William Vahand Place intersection or the Mundy Street intersection.
Traffic will be limited to speeds of 40km/h. Parking in a section of Sidney Myer Place will be affected.
"The city asks motorists and pedestrians to observe signage around the works area and apologises for any inconvenience the work may cause," Mr Martini said.
"Considerable planning has gone into this project to make sure any inconvenience is kept to a minimum and traffic operates safely during the works."
Tracks and sleepers have subsided over a long period and there is now minimal clearance between the bottom of the tram and some sections of the road pavement.
Mr Martini said the works would help preserve one of the city's most important heritage attractions.
"The city has appointed a contractor specialising in tram track repairs to undertake the work which will also involve the removal, cleaning and replacement of the historic blue stone blocks in the centre of Pall Mall," Mr Martini said.
He said the Williamson Street traffic lights would continue to operate and pedestrians would still be able to cross Pall Mall near the Shamrock Hotel and Bendigo Visitor Information Centre.
Have you signed up to the Bendigo Advertiser's daily newsletter and breaking news emails? You can register below and make sure you are up to date with everything that's happening in central Victoria.
Sign up for our newsletter to stay up to date.