A Bendigo woman was flown to Melbourne with serious, non-life-threatening injuries after a crash on the Calder Freeway on Thursday.
Macedon Ranges Highway Patrol have appealed for witnesses or dash cam footage after the crash between two cars and a truck at Gisborne.
Investigators believe a B-Double was travelling through a road works zone on the Calder Freeway when it collided with the rear of a Toyota hatchback and a Ford utility at about 3.45pm.
The driver of the hatch, a 26 year old Bendigo woman, was flown to Melbourne.
An 18-year-old Mill Park woman who was a passenger in the hatch was taken to hospital with minor injuries.
A 39-year-old Macleod man who was driving the ute was also taken to hospital with non-life-threatening injuries.
The driver of the truck, a 32-year-old Pakenham man was uninjured and stopped to assist at the scene.
Police are urging anyone who witnessed the incident and anyone who may have video footage of the collision to contact Crime Stoppers on 1800 333 000 or submit a confidential report at crimestoppersvic.com.au