Robert Dutton, 35, of Castlemaine, faced Bendigo Magistrates' Court on Tuesday on multiple charges including making threats to kill, threatening to inflict serious injury, assault with a weapon, assaulting a police officer and using threatening words in a public place.

Senior Constable Ian Kuster of Castlemaine police told the court the alleged incident occurred at 3.15pm on Monday while Mr Dutton was aboard a packed train from Bendigo to Castlemaine. 

He said two train conductors, a man and a woman, approached Mr Dutton about drinking alcohol after they allegedly found an empty bottle of Gialliano Sambuca on him.

Senior Constable Kuster said police alleged Mr Dutton stood up, became confrontational and began making abusive threats, including that he would gauge their eyes out with the empty bottle and kill them.

The court heard Mr Dutton allegedly hit the female conductor with the empty bottle, causing bruising to her arm, and allegedly violently pushed the male conductor.

The abuse allegedly continued until the train pulled up at Castlemaine train station, where police where waiting.

The court heard that after disembarking at Castlemaine, Mr Dutton allegedly tried to get back on the train by forcing the door open, breaking its lock, which meant the conductors had to lean on the door to stop him from getting back in.  

He said Mr Dutton allegedly threatened police at the train station and made further threats to the V/Line staff in front of police.

Senior Constable Kuster said both victims were in "fear of their lives" during the incident and the female conductor in particular was traumatised by the incident.

He said Mr Dutton posed an unacceptable risk for bail.

"He was unconcerned with his behaviour on a packed train, which would have terrified passengers," he said.

Defence lawyer Marcus Williams said Mr Dutton took issue with the some of the allegations made in police statements about the incident.

"He says they are exaggerated," he said. 

He said Mr Dutton had an acquired brain injury, was unemployed and recovering from a heroin and ice addiction and needed to use the train to commute to Bendigo regularly to get prescriptions for methadone.

In refusing bail, Magistrate Bruce Cottrill said it was of "significant concern" to him Mr Dutton intended to keep using the train service.

"The applicant represents a danger to witnesses and the public," he said.

"The combination of offences is extremely serious and represents what can only be described as inexplicable behaviour.

"One would expect this would have had a significant effect on each conductor and any member of the public - man, woman and child - in hearing distance."

Magistrate Cottrill remanded him to appear before Bendigo Magistrates' Court on July 28.