The luxury resort on the Great Barrier Reef scored 100 out of 100 for its rooms, service, food, location, design and activities and is the first Australian property to be voted No. 1 and only one of four properties in the survey's 25-year history to be judged perfect.
The survey of 46,476 people nominated more than 1300 winners (a minimum number of responses is required to be eligible for an award) in categories of cities, islands, cruises and airlines.
Set among eucalypts on the island's northern-most tip, the resort has a staff-to-guest ratio of 1:1 and 60 pavilions with views of the surrounding Whitsunday Islands and Coral Sea. The resort's luxurious Beach House has a 10-person dining area, pool and separate guesthouse.
The resort plays host to an outdoor performance by the Australian Ballet and the annual sailing regatta Audi Hamilton Island Race Week.
Also on the Top 100 list Taj Exotica Resort and Spa, Maldives was No.2 with a score of 99.6, and No.3 the Lodge at Kauri Cliffs, Northland, New Zealand 99.2.
Qualia Pavilions cost from $975 a night and include breakfast, non-alcoholic beverages, two-seater golf buggy, chauffeur service and return airport/marina transfers.
The resort also rates highly on TripAdvisor, the world's most popular travel review site, with the vast majority of the 305 reviews giving Qualia a five-out-of-five score.