The new branch is part of the company's Victoria-wide expansion.

Bank of Melbourne chief executive Scott Tanner said the company planned to reach the regional corners of Victoria.

"We are now covering the top five cities in the state," he said.

"We have our eyes on a few regional centres for the next branch.

"(Bendigo) is such an important regional city.

"Being the third largest (regional) city in the state, it is important for us to be there.

"We were getting quite a few people in the company who wanted to work in Bendigo.

"That gave me a lot of confidence because they wanted to be there to get involved in the local community."

Mr Tanner said at this stage, the Bendigo branches would not be focused on farming but city-based business. 

"Our focus is not on farming or agriculture, it is very much on the businesses of Bendigo," he said. 

"Our strong growth and financial performance is good news for Victoria's people and businesses.

"I am proud of the way our people are personally getting involved in making a difference in their local communities."