Workmen on site were able to contain the fuel into a bunded concrete pit.

MFB firefighters were on scene within minutes of receiving the call and ensured the fuel leak was isolated and the split product blanketed in foam.

MFB Hazmat operators used specialist equipment to monitor flammability and toxicity levels of the fuel and continued to conduct air monitoring. 

Exon Mobil representatives were also on site to give expert advice. 

The incident took 50 minutes to bring under control.

UPDATE 4.40pm: V/Line has apologised to passengers for the delay.

V/Line spokesman Colin Tyrus said they had to stop the trains until the emergency services had cleaned up the fuel.

"We organised coaches as soon as we could," he said.

"All the passengers got to where they were going.

"We apologise for the delay."

UPDATE 3.45pm: Passengers on a V/Line train travelling from Melbourne to Bendigo were delayed for more than an hour on Sunday due to aviation fuel leaks on the track.

The train left Southern Cross at 1.36pm but terminated at Sunshine just after 2pm with more than 100 people on board.

One replacement bus arrived just after 3pm, taking 60 passengers to Bendigo, and another one left just after 3.30pm to Gisborne, Woodend and Castlemaine.

About 40 passengers had to wait until 3.40pm for the trains to start running again. 

EARLIER: About 80 disgruntled V/line passengers have been waiting at Sunshine train station for bus replacements for the Melbourne-Bendigo train.

The train left Southern Cross at 1.36pm but terminated at Sunshine just after 2pm with more than 100 people on board.

One bus came just after 3pm, going to Bendigo, taking about 60 people. But it couldn't fit on all the passengers and about 80 remain.

Mothers Angela Gray and Amy-lee Purtell said they were "pissed off" by the situation.

"They haven't prioritised elderly people and those with children," Ms Purtell said.

The mothers, who both have young babies, were not put on the first bus.

"We've got no water for our babies' bottles.

"We were told to stand near the bus but weren't put on when it came."

The V/line conductor told passengers it was difficult for the company to find bus drivers on a Sunday.

He apologised to travellers for inconvenience and said it was a safety issue.

Jess Berrill, from Eaglehawk, said she felt upset about the situation.

"I'm upset because I have to get back to my daughter and I've been on a train for three hours already," she said.

"There's no shade.

"I'm hot and thirsty in the sun."

She said she was a regular passenger on the line and problems occurred frequently.

"I get a train to see my boyfriend every two weeks and this happens quite often," she said.

"There's always a hiccup."