Event co-organiser Nel Staite said more than 200 people were expected to greet him as he rides into town.

“This is going to be the hardest part for him so we want the whole community there to support and get behind him,” Ms Staite said.

The Daylesford Love Your Sister Fundraising Event is set to kick off at the Savoia Hotel in Hepburn from 6pm with special guests Jennie Brown and Bruce Millar duo, Dj Zolly Gold, Family Farm Big Band and King Stone headlining.

Ms Staite said organisers had been overwhelmed by the support and generosity of everyone in Daylesford.

“We were overwhelmed by the number of people who wanted to donate, show their support and volunteer their time,” she said.

Entry to the Savoia is $10 for adults and $5 for children with children under 5 free. 

YESTERDAY: Samuel Johnson may have pulled some fancy tricks on his trip to Bendigo - even daring to ride on top of a parked van. 

But the Daylesford-born actor, who is more than a year into his 15,000 kilometre journey across the country, wasn't there to impress a crowd. 

"These things are really hard to ride, they're strange contraptions and bikes are much better - you'd wanna have a really good reason to do what I'm doing," he said. 

"But it's not for me, it's not for my sister Connie who sent me on this epic odyssey, it's for all the women out there.

"Connie's got breast cancer, and it's too late for her, but it's not too late for all those other women who can still make checks regularly before it's too late."

He said he pedalled and pedalled and pedalled just for the "right to five minutes of people's time". 

"I've travelled 13,000 kilometres across the country, another 1000 and I will have broken the world record for the longest distance ridden on a unicycle," he said. 

"But the reason I'm travelling all year is to be that trigger point to have the conversation about health. 

"This disease is affecting our young mums and that really gets to me."

Along the way, Mr Johnson has eaten a live huntsman spider and rode his unicycle off a 60-metre high platform while attached to a bungee cord.

All to raise funds and awareness. 

Mr Johnson has already raised just shy of $1.2 million, and says it was mainly through small donations from generous community members. 

"We've raised this much not through our hard work, but because of the prevalence of the disease and the generosity of people," he said. 

"It's affecting so many people and we need to be proactive."

To donate, go to the Love Your Sister fund-raiser page at www.loveyoursister.org.au

On Saturday March 8 the Bendigo Bank will also host a Love Your Sister fund-raising cocktail dinner at The Exchange. 

 Tickets are available via the Love Your Sister Bendigo Facebook page.