The blitz operated from Gisborne to Wedderburn, with Bendigo, Echuca, Maryborough and Gisborne highway patrol police taking part.

During the operation, one vehicle was impounded after a 37-year-old Melbourne woman was detected travelling almost 40km/h above the speed limit near Elphinstone.

She was clocked at 149km/h.

One driver was fined after failing to stop at a stop sign, while two other people were fined for using mobile phones.

One driver was fined more than $500 after being detected driving an unregistered motor vehicle.

Police also issued three drivers with vehicle defect notices.

Acting Senior Sergeant Craig Gaffee from Bendigo said police in marked and unmarked vehicles took part in the operation, which ran between 6am and 9pm.

Numerous accidents, resulting in serious injuries and fatalities, have occurred on the Calder Highway and Calder Freeway and police hope a high presence on the road will encourage motorists to slow down.

Earlier this year a father of two young girls was killed after a fiery truck crash near Wedderburn, while in July an 82-year-old man died after the vehicle he was a passenger in collided with another vehicle travelling on the Calder Freeway at the Fogartys Gap Road intersection.

Acting Senior Sergeant Gaffee said police were patrolling the Calder daily.

He said police were also using stationary vehicles on the side of the road. He said the operation would be ongoing.